abstract：:A gene encoding halohydrin dehalogenase (HHDH) from Agrobacterium tumefaciens CCTCC M 87071 was cloned and expressed in Escherichia coli. To increase activity and stability of HHDH, 14 amino acid residues around the active site and substrate-binding pocket based on the structural analysis and molecular docking were se...

journal_title：Journal of industrial microbiology & biotechnology

authors： Liu ZQ,Gao AC,Wang YJ,Zheng YG,Shen YC

更新日期：2014-07-01 00:00:00

abstract：:Burkholderia cepacia complex (BCC) presence has been the cause of recalls of both sterile and non-sterile pharmaceutical products since these opportunistic pathogens have been implicated to cause infections to susceptible individuals. BCC are ubiquitous in nature, but in pharmaceutical settings the most common source ...

journal_title：Journal of industrial microbiology & biotechnology

authors： Ahn Y,Kim JM,Ahn H,Lee YJ,LiPuma JJ,Hussong D,Cerniglia CE

更新日期：2014-07-01 00:00:00

abstract：:The human epidermal growth factor receptor (HER) family plays an important role in cell growth and signaling and alteration of its function has been demonstrated in many different kinds of cancer. Receptor dimerization is necessary for the HER signal transduction pathway and tyrosine kinase activity. Recently, several...

journal_title：Journal of industrial microbiology & biotechnology

authors： Akbari V,Mir Mohammad Sadeghi H,Jafrian-Dehkordi A,Abedi D,Chou CP

更新日期：2014-06-01 00:00:00

abstract：:Somatostatin, a natural inhibitor of growth hormone (GH), and its analogs have been used in clinical settings for the treatment of acromegaly, gigantism, thyrotropinoma, and other carcinoid syndromes. However, natural somatostatin is limited for clinical usage because of its short half-life in vivo. Albumin fusion tec...

journal_title：Journal of industrial microbiology & biotechnology

authors： Ding Y,Fan J,Li W,Peng Y,Yang R,Deng L,Fu Q

更新日期：2014-06-01 00:00:00

abstract：:A metabolic network consisting of 48 reactions was established to describe intracellular processes during growth and poly-3-hydroxybutyrate (PHB) production for Cupriavidus necator DSM 545. Glycerol acted as the sole carbon source during exponential, steady-state cultivation conditions. Elementary flux modes were obta...

journal_title：Journal of industrial microbiology & biotechnology

authors： Lopar M,Špoljarić IV,Cepanec N,Koller M,Braunegg G,Horvat P

更新日期：2014-06-01 00:00:00

abstract：:The fruity odor of Chinese liquor is largely derived from ester formation. Ethyl caproate, an ethyl ester eliciting apple-like flavor, is one of the most important esters in the strong aromatic Chinese liquor (or Luzhou-flavor liquor), which is the most popular and best-selling liquor in China. In the traditional ferm...

journal_title：Journal of industrial microbiology & biotechnology

authors： Chen Y,Li F,Guo J,Liu G,Guo X,Xiao D

更新日期：2014-03-01 00:00:00

abstract：:Several unique Sus-like polysaccharide utilization loci (PULs) were identified from bacteria resident in bovine rumen microbiomes through functional screening of a fosmid library. The loci were phylogenetically assigned to the genus Prevotella within the phylum Bacteroidetes. These findings were augmented by a bioinfo...

journal_title：Journal of industrial microbiology & biotechnology

authors： Rosewarne CP,Pope PB,Cheung JL,Morrison M

更新日期：2014-03-01 00:00:00

abstract：:Natural product scaffolds remain important leads for pharmaceutical development. However, transforming a natural product into a drug entity often requires derivatization to enhance the compound's therapeutic properties. A powerful method by which to perform this derivatization is combinatorial biosynthesis, the manipu...

journal_title：Journal of industrial microbiology & biotechnology

authors： Cobb RE,Ning JC,Zhao H

更新日期：2014-02-01 00:00:00

abstract：:The use of proteomics for direct detection of expressed pathways producing natural products has yielded many new compounds, even when used in a screening mode without a bacterial genome sequence available. Here we quantify the advantages of having draft DNA-sequence available for strain-specific proteomics using the l...

journal_title：Journal of industrial microbiology & biotechnology

authors： Albright JC,Goering AW,Doroghazi JR,Metcalf WW,Kelleher NL

更新日期：2014-02-01 00:00:00

abstract：:We report microbially facilitated synthesis of cadmium sulfide (CdS) nanostructured particles (NP) using anaerobic, metal-reducing Thermoanaerobacter sp. The extracellular CdS crystallites were <10 nm in size with yields of ~3 g/L of growth medium/month with demonstrated reproducibility and scalability up to 24 L. Dur...

journal_title：Journal of industrial microbiology & biotechnology

authors： Moon JW,Ivanov IN,Duty CE,Love LJ,Rondinone AJ,Wang W,Li YL,Madden AS,Mosher JJ,Hu MZ,Suresh AK,Rawn CJ,Jung H,Lauf RJ,Phelps TJ

更新日期：2013-11-01 00:00:00

abstract：:Klebsiella oxytoca naturally produces a large amount of 2,3-butanediol (2,3-BD), a promising bulk chemical with wide industrial applications, along with various byproducts. In this study, the in silico gene knockout simulation of K. oxytoca was carried out for 2,3-BD overproduction by inhibiting the formation of bypro...

journal_title：Journal of industrial microbiology & biotechnology

authors： Park JM,Song H,Lee HJ,Seung D

更新日期：2013-09-01 00:00:00

abstract：:Acetoin, a major extracellular catabolic product of Bacillus subtilis cultured on glucose, is widely used to add flavor to food and also serves as a precursor for chemical synthesis. The biosynthesis of acetoin from pyruvate requires the enzymes α-acetolactate synthase (ALS) and α-acetolactate decarboxylase (ALDC), bo...

journal_title：Journal of industrial microbiology & biotechnology

authors： Zhang X,Zhang R,Bao T,Yang T,Xu M,Li H,Xu Z,Rao Z

更新日期：2013-09-01 00:00:00

abstract：:A formate dehydrogenase gene (fdh) originated from Candida boidinii was co-expressed in E. coli BL21 (DE3) with the cyclohexanone monooxygenase gene (chmo) cloned from Acinetobacter calcoaceticus NCIMB 9871. The co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide...

journal_title：Journal of industrial microbiology & biotechnology

authors： Zhai XH,Ma YH,Lai DY,Zhou S,Chen ZM

更新日期：2013-08-01 00:00:00

abstract：:Coumermycin A1 is an aminocoumarin antibiotic produced by Streptomyces rishiriensis. It exhibits potent antibacterial and anticancer activity. The coumermycin A1 molecule contains two terminal 5-methyl-pyrrole-2-carboxylic acid moieties and one central 3-methylpyrrole-2,4-dicarboxylic acid moiety (CPM). While the bios...

journal_title：Journal of industrial microbiology & biotechnology

authors： Novotna J,Gust B,Kulik A,Spizek J,Heide L

更新日期：2013-08-01 00:00:00

abstract：:Keratinases are proteolytic enzymes capable of degrading insoluble keratins. The importance of these enzymes is being increasingly recognized in fields as diverse as animal feed production, textile processing, detergent formulation, leather manufacture, and medicine. To enhance the thermostability of Bacillus lichenif...

journal_title：Journal of industrial microbiology & biotechnology

authors： Liu B,Zhang J,Fang Z,Gu L,Liao X,Du G,Chen J

更新日期：2013-07-01 00:00:00

abstract：:The measurements of concentration, viability, and budding percentages of Saccharomyces cerevisiae are performed on a routine basis in the brewing and biofuel industries. Generation of these parameters is of great importance in a manufacturing setting, where they can aid in the estimation of product quality, quantity, ...

journal_title：Journal of industrial microbiology & biotechnology

authors： Laverty DJ,Kury AL,Kuksin D,Pirani A,Flanagan K,Chan LL

更新日期：2013-06-01 00:00:00

abstract：:Several indigenous Saccharomyces strains from musts were isolated in the Jerez de la Frontera region, at the end of spontaneous fermentation, in order to select the most suitable autochthonous yeast starter, during the 2007 vintage. Five strains were chosen for their oenological abilities and fermentative kinetics to ...

journal_title：Journal of industrial microbiology & biotechnology

authors： Rodríguez-Palero MJ,Fierro-Risco J,Codón AC,Benítez T,Valcárcel MJ

更新日期：2013-06-01 00:00:00

abstract：:Streptomyces transglutaminase (TGase) has been widely used in food, pharmaceutical and textile industries. Streptomyces TGase is naturally synthesized as zymogen (pro-TGase), which is then processed to produce active enzyme by removing its N-terminal pro-peptide. Although the pro-peptide is essential for TGase folding...

journal_title：Journal of industrial microbiology & biotechnology

authors： Chen K,Liu S,Wang G,Zhang D,Du G,Chen J,Shi Z

更新日期：2013-04-01 00:00:00

abstract：:The aim of this work was to determine the ability of rhodococci to transform 3,5-dichloro-4-hydroxybenzonitrile (chloroxynil), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 3,5-diiodo-4-hydroxybenzonitrile (ioxynil) and 2,6-dichlorobenzonitrile (dichlobenil); to identify the products and determine their acute toxici...

journal_title：Journal of industrial microbiology & biotechnology

authors： Veselá AB,Pelantová H,Sulc M,Macková M,Lovecká P,Thimová M,Pasquarelli F,Pičmanová M,Pátek M,Bhalla TC,Martínková L

更新日期：2012-12-01 00:00:00

abstract：:Tolerance to various stresses is a key phenotype for cell catalysts, which are used widely in bioproduction of diverse valuable chemicals. Using the Rhodococcus ruber TH strain, which exhibits high nitrile hydratase activity, as the target cell catalyst for acrylamide production, we established a method to improve cel...

journal_title：Journal of industrial microbiology & biotechnology

authors： Ma Y,Yu H

更新日期：2012-10-01 00:00:00

abstract：:In this study we investigated the sensitivity of Deinococcus radiodurans to contact-free cold atmospheric plasma treatment as part of a project to establish new efficient procedures for disinfection of inanimate surfaces. The Gram-positive D. radiodurans is one of the most resistant microorganisms worldwide. Stationar...

journal_title：Journal of industrial microbiology & biotechnology

authors： Maisch T,Shimizu T,Mitra A,Heinlin J,Karrer S,Li YF,Morfill G,Zimmermann JL

更新日期：2012-09-01 00:00:00

abstract：:Selection of the ideal microbe is crucial for whole-cell biotransformations, especially if the target reaction intensively interacts with host cell functions. Asymmetric styrene epoxidation is an example of a reaction which is strongly dependent on the host cell owing to its requirement for efficient cofactor regenera...

journal_title：Journal of industrial microbiology & biotechnology

authors： Kuhn D,Bühler B,Schmid A

更新日期：2012-08-01 00:00:00

abstract：:The Red recombinase system, the most convenient genetic tool applied in Escherichia coli and other bacteria, was introduced for gene replacement in Klebsiella pneumoniae. The novel K. pneumoniae gene replacement system comprised the Red and FLP recombinases expression vector pDK6-red and pDK6-flp, and linear DNA fragm...

journal_title：Journal of industrial microbiology & biotechnology

authors： Wei D,Wang M,Shi J,Hao J

更新日期：2012-08-01 00:00:00

abstract：:Lasiodiplodan, an exopolysaccharide of the (1→6)-β-D: -glucan type, is produced by Lasiodiplodia theobromae MMPI when grown under submerged culture on glucose. The objective of this study was to evaluate lasiodiplodan production by examining the effects of carbon (glucose, fructose, maltose, sucrose) and nitrogen sour...

journal_title：Journal of industrial microbiology & biotechnology

authors： Alves da Cunha MA,Turmina JA,Ivanov RC,Barroso RR,Marques PT,Fonseca EA,Fortes ZB,Dekker RF,Khaper N,Barbosa AM

更新日期：2012-08-01 00:00:00

abstract：:A halotolerant isolate Bacillus sp. L1 producing extracellular cellulase was isolated from Yuncheng, China. Production of the enzyme started from mid-exponential phase of bacterial growth and reached a maximum level during the post-stationary phase. The cellulase was purified to homogeneity with molecular mass of 45 k...

journal_title：Journal of industrial microbiology & biotechnology

authors： Li X,Yu HY

更新日期：2012-08-01 00:00:00

abstract：:The importance of selecting optimal yeast strains for research or industrial applications is often underestimated. For example, utilizing a strain background that already provides the desired stress tolerance or nutrient utilization profile can eliminate costly strain optimization. Yeast culture collections can provid...

journal_title：Journal of industrial microbiology & biotechnology

authors： Boundy-Mills K

更新日期：2012-05-01 00:00:00

abstract：:A novel lipolytic enzyme was isolated from a metagenomic library after demonstration of lipolytic activity on an LB agar plate containing 1% (w/v) tributyrin. A novel esterase gene (estIM1), encoding a lipolytic enzyme (EstIM1), was cloned using a shotgun method from a pFosEstIM1 clone of the metagenomic library, and ...

journal_title：Journal of industrial microbiology & biotechnology

authors： Ko KC,Rim SO,Han Y,Shin BS,Kim GJ,Choi JH,Song JJ

更新日期：2012-05-01 00:00:00

abstract：:In this article, we firstly report a highly alkali-tolerant fungal β-mannanase from Humicola insolens Y1. The full-length cDNA of the β-mannanase, designated as man5A, has an open reading frame of 1,233 bp that encodes a 411-amino acid polypeptide (Man5A) with a calculated molecular mass of 42.3 kDa. The deduced seque...

journal_title：Journal of industrial microbiology & biotechnology

authors： Luo H,Wang K,Huang H,Shi P,Yang P,Yao B

更新日期：2012-04-01 00:00:00

abstract：:The overall objective of this study is to evaluate the morphological [scanning electron microscopy (SEM)], physicochemical [differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), chemical composition analysis, Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR)], and bi...

journal_title：Journal of industrial microbiology & biotechnology

authors： Cabrera-Padilla RY,Lisboa MC,Fricks AT,Franceschi E,Lima AS,Silva DP,Soares CM

更新日期：2012-02-01 00:00:00

abstract：:Lactobacillus casei Zhang is a widely recognized probiotic bacterium, which is being commercially used in China. To study the gene expression dynamics of L. casei Zhang during fermentation in soymilk, a whole genome microarray was used to screen for differentially expressed genes when grown to the lag phase, the late ...

journal_title：Journal of industrial microbiology & biotechnology

authors： Wang JC,Zhang WY,Zhong Z,Wei AB,Bao QH,Zhang Y,Sun TS,Postnikoff A,Meng H,Zhang HP

更新日期：2012-01-01 00:00:00

abstract：:The industrial crops of microalgae use processes calling upon the presence of parts of metal nature such as steel 316L type. The goal of this study is to test the electrochemical behavior of this material in a marine culture of microalgae. Porphyridium purpureum was used under a photoperiod of alternation darkness/lig...

journal_title：Journal of industrial microbiology & biotechnology

authors： Djemai-Zoghlache Y,Isambert A,Belhaneche-Bensemra N

更新日期：2011-12-01 00:00:00

abstract：:In order to improve the fermentative efficiency of sugar maple hemicellulosic hydrolysates for fuel ethanol production, various methods to mitigate the effects of inhibitory compounds were employed. These methods included detoxification treatments utilizing activated charcoal, anion exchange resin, overliming, and eth...

journal_title：Journal of industrial microbiology & biotechnology

authors： Stoutenburg RM,Perrotta JA,Nakas JP

更新日期：2011-12-01 00:00:00

abstract：:Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid...

journal_title：Journal of industrial microbiology & biotechnology

authors： Choi O,Wu CZ,Kang SY,Ahn JS,Uhm TB,Hong YS

更新日期：2011-10-01 00:00:00

abstract：:There have been numerous developments in ethanol fermentation technology since the beginning of the new millennium as ethanol has become an immediate viable alternative to fast-depleting crude reserves as well as increasing concerns over environmental pollution. Nowadays, although most research efforts are focused on ...

journal_title：Journal of industrial microbiology & biotechnology

authors： Puligundla P,Smogrovicova D,Obulam VS,Ko S

更新日期：2011-09-01 00:00:00

abstract：:Huperzia serrata is a producer of huperzine A (HupA), a cholinesterase inhibitor (ChEI). Over 120 endophytic fungi were recovered from this plant and screened for Hup-A and nine were found. These nine represented seven different fungal genera with the most significant producer being Shiraia sp. A total of 127 endophyt...

journal_title：Journal of industrial microbiology & biotechnology

authors： Wang Y,Zeng QG,Zhang ZB,Yan RM,Wang LY,Zhu D

更新日期：2011-09-01 00:00:00

abstract：:Study of the distribution of the oxygen mass transfer coefficient, k (l) a, for a stirred bioreactor and simulated (pseudoplastic solutions of carboxymethylcellulose sodium salt) bacterial (P. shermanii), yeast (S. cerevisiae), and fungal (P. chrysogenum free mycelia) broths indicated significant variation of transfer...

journal_title：Journal of industrial microbiology & biotechnology

authors： Caşcaval D,Galaction AI,Turnea M

更新日期：2011-09-01 00:00:00

abstract：:An acid proteinase from Monascus purpureus No. 3403, MpuAP, was previously purified and some characterized in our laboratory (Agric Biol Chem 48:1637-1639, 1984). However, further information about this enzyme is lacking. In this study, we investigated MpuAP's comprehensive substrate specificity, storage stability, an...

journal_title：Journal of industrial microbiology & biotechnology

authors： Lakshman PL,Tachibana S,Toyama H,Taira T,Suganuma T,Suntornsuk W,Yasuda M

更新日期：2011-09-01 00:00:00

abstract：:Experiments based on a 2(3) central composite full factorial design were carried out in 200-ml stainless-steel containers to study the pretreatment, with dilute sulfuric acid, of a sugarcane bagasse sample obtained from a local sugar-alcohol mill. The independent variables selected for study were temperature, varied f...

journal_title：Journal of industrial microbiology & biotechnology

authors： Canilha L,Santos VT,Rocha GJ,Almeida e Silva JB,Giulietti M,Silva SS,Felipe MG,Ferraz A,Milagres AM,Carvalho W

更新日期：2011-09-01 00:00:00

abstract：:Biocatalytic hydrocarbon oxyfunctionalizations are typically accomplished using oxygenases in living bacteria as biocatalysts. These processes are often limited by either oxygen mass transfer, cofactor regeneration, and/or enzyme instabilities due to the formation of reactive oxygen species. Here, we discuss an altern...

journal_title：Journal of industrial microbiology & biotechnology

authors： Utkür FO,Gaykawad S,Bühler B,Schmid A

更新日期：2011-08-01 00:00:00

abstract：:A newly isolated strain Penicillium sp. GXU20 produced a raw starch-degrading enzyme which showed optimum activity towards raw cassava starch at pH 4.5 and 50 °C. Maximum raw cassava starch-degrading enzyme (RCSDE) activity of 20 U/ml was achieved when GXU20 was cultivated under optimized conditions using wheat bran (...

journal_title：Journal of industrial microbiology & biotechnology

authors： Lin HJ,Xian L,Zhang QJ,Luo XM,Xu QS,Yang Q,Duan CJ,Liu JL,Tang JL,Feng JX

更新日期：2011-06-01 00:00:00